Conklin Lab Protocols

Homogenization and Solubilization of RASSLs

Immunoprecipitation of RASSLs

Immunoblotting of RASSLs

Detection of Flag-tagged RASSLs by ELISA in Tissue Culture Cells

FACS Detection of RASSLs in Blood Cells

IP- Western of HA-tagged Proteins

X-gal Staining Protocol

3H Thymidine Incorporation Assay for Rat-1a Cells

Ed_and_Nathans_ABI7900HT_qPCR_Protocol_R._20080216.doc: qPCR setup and analysis protocol

mESCs_feeder-free_transitionFINAL.doc Transitioning G4 mESCs to feeder free conditions

Information about the Tetracycline Regulated Systems

Tet Systems

Mouse Protocols

ES_Cell_Culture_for_Blastocyst_Injection.doc

Rapid Mouse Activity Assay

Mouse Heart Harvest for DNA Arrays

Doxycycline Administration to Mice

Mouse Tail DNA Analysis Protocol

Administration of Spiradoline to Mice Expressing RASSLs Ro1 or Ro2

Gene Expression Analysis of Heart Tissue

Mouse Heart Tissue Harvest for DNA Microarrays

ES_Cell_Culture_for_Blastocyst_Injection.doc: ESC Blastocyst Injection Protocol

IHC Protocols

Immunohistochemistry_for_Flag_Antibody_on_Bone_Sections_in_Paraffin_R._20071105.pdf: FLAG IHC

Immunohistochemistry_for_Osterix_Antibody_R._20071102.pdf: OSX IHC

Immunohistochemistry_for_Osteocalcin_Antibody_R._20071102.pdf: OC IHC